Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Alkali Scientific QuickSilver TBS-T Buffer Powder, 100 Pouches
QuickSilver™ Buffer Powder packs offer extreme convenience and will change the way you prepare buffers in your lab. They are quick, and easy to use. Convenient premeasured pouches make 1L of 1X buffer. There is no pH adjusent needed and they offer a long shelf life. They come supplied with preprinterd bottle labels for easy identification. 100 pouches per pack.
Teknova 1M Tris-HCl, pH 9.0. 1000mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.
National Diagnostics TRIS-GLYCINE-SDS PAGE BUFFER (
Tris-Glycine-SDS PAGE Buffer (10X), Ultra-pure concentrated standard running buffer for SDS-PAGE. Formulated with 18 MegOhm water. 0.2 micron filtration, 4 LT
Sigma Aldrich Fine Chemicals Biosciences Dulbeccos Modified Eagles1L
DULBECCOS Dulbeccos Modified Eagles1L
National Diagnostics TRIS-GLYCINE-SDS PAGE BUFFER (
Buffer; National Diagnostics; Tris-Glycine-SDS PAGE (10X); Formulated w/18 Megohm water and 0.2u filtration; Saves time and improves results; Size: 1L
STEMCELL Technologies RoboSep™ Human CD11b Positive Selection and Depletion Kit, For processing: 1 x 10^9 cells
EasySep™ kit with RoboSep™ buffer and RoboSep™ filter tips
National Diagnostics PROTOGEL RESOLVING BUFFER 1 LT
ProtoGel Resolving Buffer (4X), 4X Laemmli Tris-HCl resolving gel buffer for clear, reproducible SDS-PAGE, 1 LT
![Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 50 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75030-50.0.JPG-250.jpg)
Research Products International Corp HEPES, Free Acid [N-(2-Hydroxyethyl) piperazine N'-(2-ethanesulfonic acid)], 50 Grams
HEPES, Free Acid (4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid), is a zwitterionic buffer.
In the life science field, HEPES, Free Acid, is primarily utilized as a buffering agent to maintain a stable pH in various experimental procedures. It is particularly effective in the pH range of 6.8 to 8.2, making it suitable for applications requiring slightly acidic to neutral pH conditions.
HEPES, Free Acid, is commonly used in cell culture media, protein purification, and enzyme assays where a specific pH range needs to be maintained. Its buffering capacity and stability within the desired pH range make it valuable in these applications.
One of the significant advantages of HEPES, Free Acid, is its compatibility with biological systems and enzymes. It exhibits minimal interference with enzymatic activity and other biological processes, ensuring reliable and accurate results.
Quality Biological Inc Tris HCl (2 M) pH 6.8 500mL
Tris solutions are formulated into working concentrations for preparing the stacking and resolving portions of native or SDS-PAGE gels using discontinuous buffer systems according to Laemmli (1970) or Ornstein and Davis (1959). Tris HCl Buffer is compatible with many enzymes in molecular biology (e.g. DNA modifying enzymes). Use the Tris-HCl 0.5 M (pH 6.8) buffer for stacking gels. Use Tris-HCl 1.5 M (pH 8.8) buffer for resolving gels. Use Tris-HCl 1M pH 7.0-7.5 for DEAE Dextran-mediated transfection of non-adherent and adherent cells.Manufactured in an ISO 90012015 certified facility.
Quality Biological Inc TE pH 7.4 100mL
TE is composed of buffering agent Tris and chelating agent EDTA. EDTA prevents degradation of DNA and RNA by chelating magnesium (or other divalent metal ions). Together this Tris-EDTA based solution solubilizes DNA or RNA while protecting it from degradation. Filtered through a 0.22 micron membraneDnase Rnase and Protease tested Manufactured in an ISO 90012015 certified facility.
Abcam PBS Tablets (1000 ml), 10TABLET
MW 411.04 Da. One of the most commonly used buffers provided in a convenient, tablet form. PBS is isotonic and non-toxic to cells and has the ability to maintain their osmolarity thereby making the buffer suitable for washing procedures in cell cultures and for immunoassays.
The product is subject to the following: Abcam Restricted Use Statement
Quality Biological Inc Tris (1 M) pH 9.8 1000mL
Suitable for molecular biology protein chemistry and biochemical applications.DNase RNase and Protease tested.Filtered through a 0.22 micron membraneDnase Rnase and Protease tested Manufactured in an ISO 90012015 certified facility.
Teknova 1M Tris-HCl, pH 8.5. 1000 mL, Sterile.
Tris (tris(hydroxymethyl)aminomethane) is used as a component ofbuffer solutions, especially for solutions ofnucleic acids. The useful buffer range for tris (pH 7–9) is similar to the pH of most living organisms. This, and its low cost, make tris one of the most common buffers in the biology/biochemistry laboratory.